The centre-back was withdrawn after 56 minutes for the 2-1 reverse.
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Slot said afterwards: “I don’t know if it is a big injury, but what I do know is he limped a little bit and when I asked him he said he felt his quad a little bit.
“Then, for me, all alarm bells go off because in another sprint you never know if that is going to go good.
“It was also already a substitution I had in mind, maybe for a few minutes or a little bit later, because the right centre-back had a lot of ball possession and we needed to create from that position.
“It might be OK that he went off early enough but it was clear I had to take him off because he was limping.”