Rosenior was upset with Tierney positioning himself in the middle of the Blues' huddle before Saturday's game against Newcastle.
"If Paul had focused more on his job, which was to make the right decision, we'd have a penalty today," Rosenior said after Saturday's 1-0 defeat. "I don't think anyone can say that (Nick) Woltemade doesn't kick (Cole) Palmer down in the box.
"I'll be speaking to PGMOL and the refs to get an understanding of why that happened today," he added. "We were told that in the rulebook you can be where you want and it's about timing.
"I'm disappointed. I'm going to make it clear. I want to protect my players and I'm respectful to the game.
"Before the game we had a meeting with the referee. My assistant goes in and the first thing (Tierney) talks about is our huddle. We're not being disrespectful to the opposition."
Former Premier League assistant referee referee Darren Cann told BBC Sport: "As it was Newcastle's kick-off, the referee (took) control of the ball on the halfway line and was there to prevent any potential issues.
"When it is their kick-off, there is no particular issue. However, when their opponents have to kick off, there is potential for confrontation."