The former Manchester City defender made 96 caps for the Three Lions with his debut against Spain coming in 2011 when he was just 21 years old.
His announcement brings an end to a 15-year career with England which included reaching consecutive European Championship finals.
He revealed his decision to end his international career on Tuesday in what was an announcement nobody expected.
“I am sad to be making this decision, but I’m also very proud of what I've achieved with England,” Walker said.
“To represent my country at five major tournaments, to get to the finals that we've reached and to be surrounded by this England team, especially the players and the managers that I've had the privilege of working under, has been a great honour.
“But today is the time that comes to an end. It's good for me to have a little bit of closure to my career on the international stage.”
Tuchel says Walker should be filled with pride
England boss Tuchel said: “I know all England fans will join me in congratulating Kyle on an incredible international career.
“Although I only worked with him for a short period of time, I was always aware that he was one of England’s greats who fully embraced the highest honour of representing his country.
“An international career spanning 14 years and five major tournaments is testament to Kyle’s dedication. He can look back on his time with the Three Lions with great pride.”
Walker, whose only senior international goal came against Ukraine in 2023, now features for Burnley in the Premier League as he prepares to turn 36 years old in May. He faces relegation with the club this season as the club try to climb out of the relegation zone.