The Argentinian was substituted due to injury in the Copa del Rey win over Malaga.
Messi scored Barcelona's fourth goal in a 4-2 away win that carried the Catalans through to the semi-finals 6-4 on aggregate.
He was substituted two minutes later, in the 82nd minute, but Barcelona stress he is not carrying a significant injury.
Assistant head coach Jordi Roura said on fcbarcelona.com: "We brought Messi off the as a preventative measure. The adductor in his right leg was giving him problems. It's nothing serious."