MK Dons will host AFC Wimbledon in the first round of the Capital One Cup.
The grudge match will be the second time the two rivals have met since Wimbledon moved 54 miles to Milton Keynes in 2003 and AFC Wimbledon were formed in protest.
Their previous meeting saw the MK Dons triumph 2-1 in an FA Cup second round game.
Elsewhere, newly-promoted Cambridge United, who won promotion to League Two with a Conference play-off final victory over Gateshead, will face 2011 winners Birmingham City at St Andrews.
Conference champions Luton will take on League One Swindon, winners of the League Cup back in 1969, at Kenilworth Road.
CAPITAL ONE CUP FIRST ROUND DRAW
Leeds United v Accrington Stanley
Rotherham United v Fleetwood Town
Port Vale v Hartlepool United
Morecambe v Bradford City
Barnsley v Crewe Alexandra
Burton Albion v Wigan Athletic
York City v Doncaster Rovers
Sheffield United v Mansfield Town
Reading v Newport County
Bristol City v Oxford United
Brighton & Hove Albion v Cheltenham Town
Luton Town v Swindon Town
Dagenham & Redbridge v Brentford
Wolverhampton Wanderers v Northampton Town
Yeovil Town v Gillingham
Milton Keynes Dons v AFC Wimbledon
Plymouth Argyle v Leyton Orient
Birmingham City v Cambridge Untied
Southend United v Walsall
For the big stories and the banter follow us on Twitter: @tribalfootball