The Potters will take on the Dynamo at BBVA Compass Stadium on July 24 as part of their preseason preparations for the 2013/14 Barclays Premier League campaign, marking the return of former Dynamo defender Geoff Cameron to his old club.
"We are pleased to be hosting an historic English Premier League club at BBVA Compass Stadium as part of this growing tradition," said Dynamo President Chris Canetti.
"This event showcases the international element of soccer, provides a fun and entertaining night for fans, and offers a terrific charitable angle for the community."
Stoke chief executive Tony Scholes added: "The tour to the United States was extremely successful last year, first and foremost in achieving our primary objective of helping to prepare the squad for the season ahead. The transfers of Geoff Cameron and Brek Shea subsequently presented opportunities for us to play matches against their former clubs which is where we will be heading."