The pair were left out of the Roma side on Sunday.
Prandelli said: "Osvaldo and De Rossi? Zdenek Zeman obviously had fresher players at his disposal. I don't really have anything in particular to say about it.
"De Rossi and Osvaldo have always been at my disposal on national duty and they've been able to train twice a day. Knowing Zeman, it may have been a type of provocation.
"We have two players who are not in great psychological shape in De Rossi and Balotelli. We'll need to work on their pride."