Osvaldo has been dropped from the squad after breaking the code of ethics by repeatedly insulting Roma Coach Aurelio Andreazzoli.
"Daniel is part of our group and it's always a shame when a teammate isn't there," said Chiellini.
"The decisions are made by the Coach and we will wait for Osvaldo with arms outstretched if Prandelli in future decides to bring him back. He always behaved very well with us.
"The code of ethics was an approach started three years ago and we know that as players we have great responsibility, as we are representing an entire country. Of course we have to set a good example for children.
"A positive result in Prague would allow us to secure qualification, but we must be very wary, as a negative result could put us right back in the thick of the battle."