The Barcelona midfielder was spotted stamping on the head of a floored Real defender Pepe during Sunday's El Clasico - but he has escaped punishment.
"I wonder what would have happened if the incident between Pepe and Busquets had been the other way around," snapped Ancelotti.
"Real Madrid isn't going to file a complaint because that's not the way the club goes about things."
Ancelotti also took aim at ref Undiano Mallenco.
"I think he played a decisive role. We were unlucky with the Sergio Ramos decision because it was an unfair red card and that's why we've appealed. We've been unlucky with Undiano in both Clásicos."
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