Villa's disgruntled fans accosted captain Micah Richards, protesting against the club's poor form, but then applauded Wycombe off the field.
Ainsworth made a point of thanking the travelling supporters for respecting his side's gritty performance.
"It's great and just fantastic for the Villa fans to clap us off, because they are in a bit of disarray at the moment," said Ainsworth.
"So for them to have the respect to do that for my boys, I'd just say thank you.
"We'll see what happens up at Villa Park, we'll be giving our all again.
"With a little bit of luck you never know what happens in this competition."