Watford have lost their appeal regarding the red card received by Christian Kabasele against Wolves.
The 28-year-old got a red due to a VAR intervention, as the referee had originally given him a yellow.
It means he will miss the club's next two games.
While the Hornets claimed a 2-1 success in the game, they will be hoping they can cope without the presence of their key defender in the coming matches.
Watford have enjoyed a good run of late, as they have 10 points from their past four league matches.