Marca reports Varane is now a doubt ahead of this week's Champions League semi-final second leg away to Chelsea.
The World Cup winner was forced off at half time of the Osasuna win due to some discomfort in his right leg.
The Frenchman will undergo tests on Sunday, but his involvement against Chelsea now looks complicated.
This presents Zinedine Zidane with yet another setback and the coach may now have to rush Sergio Ramos back into the XI sooner than he might have otherwise liked to.
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