The Blues drew 0-0 with Brighton on Monday, with the game delayed by 15 minutes after supporters protested about the Super League proposal out the front of Stamford Bridge.
"I was affected so I think the players were affected," Tuchel told BBC Sport.
"We talk of nothing else but Super League before the match. Nobody asked about the match before. It is like this. You have to accept the distraction.
"We tried to create an atmosphere to win the game but could not. You really think there will be no more questions for me on this? I don't know."
It was revealed just before kick-off that Chelsea intend to withdrew from the proposed competition.
Manchester City have also formally started proceedings to withdraw.