Ethan Laird, Di'Shon Bernard, Dylan Levitt, D'Mani Mellor, Largie Ramazani and Ethan Galbraith all made their debuts in the loss in Kazakhstan.
Speaking ahead of Thursday's clash til AZ Alkmaar, Tuanzebe reserved some praise for his young teammates, who could once again feature in the competition.
"I think they played good football and showed qualities of being Man Utd players," Tuanzebe said.
"Unfortunately, we weren't able to win the game, but I was more than happy with a lot of the lads' performances.
"They have big characters and courage to come out and perform in a difficult ground - there were so many variables and we were unlucky that we didn't win the game."
On facing AZ, the defender added: "Although we're already qualified, we want to put out a strong side and win the game.
"Remembering the [away] game against Alkmaar, they were a decent side and played decent football. Normally, Dutch sides are very technical. We want to win the last game at home and win the group."