Tottenham Hotspur have begun proceedings to withdraw from the proposed European Super League.
Manchester City and Chelsea announced they would leave the new competition earlier on Tuesday.
They were followed later on by the other four English clubs involved - Spurs Manchester United, Liverpool and Arsenal - in withdrawing from the breakaway league.
In a statement posted on the club's official website, chairman Daniel Levy said: "We regret the anxiety and upset caused by the ESL proposal.
"We felt it was important that our club participated in the development of a possible new structure that sought to better ensure financial fair play and financial sustainability whilst delivering significantly increased support for the wider football pyramid.
"We believe that we should never stand still and that the sport should constantly review competitions and governance to ensure the game we all love continues to evolve and excite fans around the world.
"We should like to thank all those supporters who presented their considered opinions."