Sampdoria coach Claudio Ranieri fired a warning at his players after their defeat to Atalanta on Sunday.
Ranieri plans to ring the changes for the derby with Genoa.
"I don't want the team to sit down," Ranieri said.
“Anyone who is not with me, will not play. For us, this is the match of the year, we will arrive ready."
Samp currently sit in tenth position on the Serie A table.