Sancho, who did come on in the Carabao Cup final on Sunday against Newcastle, missed out on the game on Wednesday.
While United did win 3-1 in his absence, ten Hag stated pre-game that Sancho should not be out for too long.
"No, it's about using the squad and until this moment, we use everyone," Ten Hag told MUTV when asked about rotating his team.
"They all have to be ready to play, so obviously we make six changes, but the rules and principles are the same
"One is ill [Sancho], the other [Luke Shaw] is a small injury, I hope it's not too bad.
“We'll have to see how it develops to Sunday, but he's not available for tonight. We know we have good players in the squad and we can replace that."