Real Madrid midfielder Toni Kroos was forced off during yesterday's 2-2 draw with Valencia.
It means the German is a doubt for Real's Champions League semifinal second-leg clash with Juventus.
"Kroos had a blow to his hip and we are going to evaluate it tomorrow. It doesn't seem to be a muscle problem and we are quite positive about it," said Ancelotti.
"The same with Karim, who has a little problem from a hit and I think he will be ready for Wednesday.
"Varane had a fever over the course of the night and was quite tired. Now he doesn't have the fever and I believe he will be able to train normally tomorrow."