Tottenham striker Harry Kane rapped team-mates as England slid to their first qualifying defeat in ten years.
Skipper Kane gave Gareth Southgate's men a perfect start with a fifth-minute penalty before the Czech Republic's pair Jakub Brabec and Zdenek Ondrasek rocked the Three Lions.
Kane fumed: “It was down to us. We went ahead early and had the perfect start to an away game. After that we were sloppy with the ball, they scored not long after, and we just didn't move it as quickly as we normally do.
“We weren't fighting, we weren't pressing as much as we do. We gave the ball away too cheaply, especially in the first half.
“We had a couple of chances to put the game to bed but didn't and unfortunately gave one away at the end. It's a bit of a wake-up call."