Italy coach Roberto Mancini has included Juventus captain Giorgio Chiellini in his Nations League squad.
Italy will face Spain in the Nations League semi-final at the Stadio Giuseppe Meazza in Milan on October 6.
The winners of that match will then play the Final against either France or Belgium, while the losers go into the third place final.
Both those games are on Sunday October 10.
Italy squad:
Goalkeepers: Gianluigi Donnarumma (Paris Saint Germain), Alex Meret (Napoli), Salvatore Sirigu (Genoa)
Defenders:Francesco Acerbi (Lazio), Alessandro Bastoni (Inter), Leonardo Bonucci (Juventus), Giorgio Chiellini (Juventus), Giovanni Di Lorenzo (Napoli), Emerson Palmieri (Lyon), Rafael Toloi (Atalanta)
Midfielders: Nicolò Barella (Inter), Bryan Cristante (Roma), Jorginho (Chelsea), Manuel Locatelli (Juventus), Lorenzo Pellegrini (Roma), Matteo Pessina (Atalanta), Marco Verratti (Paris Saint Germain)
Forwards:Domenico Berardi (Sassuolo), Federico Bernardeschi (Juventus), Federico Chiesa (Juventus), Ciro Immobile (Lazio), Lorenzo Insigne (Napoli), Giacomo Raspadori (Sassuolo).