Two early goals from Massimiliano Allegri's side set up the victory for the Bianconeri who recoiled from their penalty shootout defeat to Melbourne Victory on Saturday night.
Paulo Dybala and Medhi Benatia found the back of the net inside 15 minutes for the reigning Serie A champions before half-time substitute Erik Lamela cut the deficit for Spurs with a well-taken goal in the 67th minute.
The opening goal of the contest came when young Spurs defender Dominic Ball gave his namesake away cheaply to Roberto Pereyra who set it up kindly for Dybala to wrap his trusty left foot around and fiercely beyond Michel Vorm.
The second goal came less than 10 minutes later when Juventus new boys Miralem Pjanic and Medhi Benatia combined, allowing the latter to provide a lovely looping header from the former's lofted, angled ball. Both players were on debut for the Bianconeri after joining from Roma and Bayern Munich respectively.
Spurs pulled one back with just under 25 minutes remaining when Victor Wanyama, in his first outing for the North Londoners, won the ball with a strong tackle which fell for Shayon Harrison who knocked it into the path of Erik Lamela. The Argentine picked his spot and fired a well-taken effort beyond Neto.
Earlier, a deadly looking Dybala raced onto a neat ball over the top before hammering a rasping volley at Vorm who did well to parry it away.
The 33rd minute provided a penalty shoot for Spurs when Will Miller was felled by Benatia but referee Ben Williams waved away the protests.
20 minutes later, Hernanes played a cleverly flighted ball into the path off Pjanic who fired his half-volley over the target as Juventus pressed for a third.
After Tottenham struck, Lamela had a great chance to equalise with his second but hit his attempt straight at a grateful Neto who palmed it to safety.
The Brazilian gloveman was then at the top of his game to deny Harrison late in the piece as the Premier League outfit missed on an injury-time leveller as the Italians attempted to preserve their lead.
In the end, Juventus held on for a win in what was a solid test for all involved, particularly the plethora of inexperienced youngsters who were given their chance by both Allegri and Tottenham boss Mauricio Pochettino.
2016 International Champions Cup - Game 2