Manchester United's owners have put up another 9.5 million shares, with a value of $186.86m (£137.12m), for sale.
United notified the New York Stock Exchange of the sale on Tuesday evening, in the names of club directors Kevin Glazer and Edward Glazer.
The statement makes it clear the club "will not receive any proceeds from the sale".
That followed the collapse of the European Super League project which saw United co-chairman Joel Glazer heavily involved in the negotiations.
In March, Avram Glazer sold £70m worth of shares in the club.
The sale represents 8% of the family's total combined ownership, although they will still hold a combined 69% of the shares in United and sources say they remain committed to the club.
It is almost certain the shares will be sold at a price lower than the Stock Market value because of the sheer number being sold.
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