Dalot, 20, has been sidelined since he came off in the second-half of United's 1-0 defeat at Newcastle on October 6, but resumed full training last week and could start against League Two Colchester.
"Maybe Diogo [will be back] after he's had a week of training but probably no-one else," manager Ole Gunnar Solskjaer said. "We've got a squad and we've got to rotate and we did that on Thursday [against AZ Alkmaar], so we're going make changes again because there are so many games.
"I hope and I think the team I'm going to put out is going to be a strong enough one to win a game. At home against a League Two side you're big favourites and we've got to accept that but we have to go out there and earn it.
"It's going to be strong enough to create chances and hopefully score goals."
Possible United XI: Romero, Dalot, Tuanzebe, Jones, Young, Garner, Matic, Pereira, Chong, Mata, Greenwood