The Australia international has been ruled out of the Foxes' final game of the season after suffering suspected knee ligament damage in Saturday's disappointing 3-1 home defeat to fellow strugglers Sheffield Wednesday.
Ahead of his side's trip to the Britannia Stadium, Holloway said: "He is certainly not going to feature against Stoke.
"We won't have a real idea about the extent of Patrick's injury until later in the week, but I have to say it is not looking good."