The staff were summoned to an 11am meeting on Monday with administrator David Elliot of Wilson Field Ltd, where the news that money has run out was confirmed.
There remains a glimmer of hope for Gretna, who have been relegated from the Clydesdale Bank Premier League, with one potential buyer still looking at the club.
Director of football Mick Wadsworth, who loses his job, said: "All the staff have been made redundant this morning.
"We're told there is still one interested party, but because there are no funds at the moment to carry on then everybody has been made redundant.
"So the assumption from that, for most of us, is that it's the end of the line regarding employment at Gretna."