“It's three times now at the end of the game, I'm getting a bit frustrated," Defour said after suffering yet more Arsenal heartbreak.
“I didn't see the penalty, I was on the bench, at some point I saw the referee pointing to the penalty spot and I thought 'not again'."
But Defour also told the Lancashire Evening Telegraph: "They were a bit surprised we played football, but even when they pressed us we stayed calm, we played out and got some combinations.
“I think we're proving every game over and over again that we deserve it. I don't think we've stolen any points, they were all deserved.
“It was a bit of a blow because we didn't deserve to lose."
The Under-18s' coach, who was a scoring member of the Everton side that beat Blackburn Rovers in the 1998 final, said: “We went away to Prague in the summer and played in a tournament there, but even then the lads were saying 'we can't wait for the Youth Cup'.
“That was pre-season, so everyone of the lads have been itching to get tucked into this tournament.
“It's been mentioned on a number of occasions and the lads who have been up in the under-23s have been chipping away at me and saying 'we'll be back for the Youth Cup, we're available', so they are all looking forward to it.
“It's a great tournament – one I have great memories of – and we are going into the game in good shape after a couple of decent results.
“It's an opportunity for the lads to play at Turf Moor, where ultimately they all want to go on and play, and, of course, we want to get through.
“It should be a close contest, but it's a tie where we really fancy our chances. It's an exciting game for us."