The Championship side go into the tie as underdogs but Struber feels his players will give a good account of themselves.
"It is a big game for me and my boys and the club," said the Austrian.
"We play against a special team with special key players and it is a nice feeling for everyone to play against Chelsea.
"This is my first time at Stamford Bridge and against an opponent on this level. It is a special feeling and challenge, but I like challenges at this level and we have to be brave on the field.
"I trust my boys and my whole squad that we can play a good game. After that, we will see with the result."
While Barnsley have started slowly in the league, losing their first two games, Struber remains upbeat about the prospects of his team.
"It is always important that you believe and I believe in my boys," the 43-year-old added.
"It is the best place for education, but I hope we do not have moments running against us, but a situation to show we are professional players, but to show Chelsea that Barnsley is not only a nice place, but also ready for good football."