It is understood Villa captain Micah Richards was required to intervene in order to calm the heated verbal exchange.
A fan told Aston Villa fanpage Vitalfootball.co.uk both Guzan and Lescott were engaged in a competition to see who could spit their chewing gum closest to the pitch and responded angrily when questioned about their behaviour by fans.
"I object to two senior players spending 90 minutes laughing and joking on the bench and littering the pitch with their chewing in some sort of childish game," the eyewitness said.
"The guy next to me knocked on the dugout, they both turned round, we said they were disgraceful and obviously didn't care.
"With that Guzan told him to 'f**k off', I knocked the dugout and told Lescott he is supposed to be Villa to which he replied: 'I am'.
"He turned away, I knocked the dug out again and said: 'why are you not acting like one?' with which he said: 'f**k off'.
"When Micah came round to us he placated the situation and understood our frustrations.
"Fair play to Micah for calming the situation.
"It's breaking my heart what is happening at our great club.
"We are all aware of the state our great club is in but we are the club and should be listened to.
"I have written to the club this morning and await their reply, if any will come I do not know."