Assistant manager Frank Barlow takes charge on a caretaker basis, starting with Sunday's trip to Tottenham.
A club statement read: "Wigan Athletic have announced this morning that they have parted company with manager Chris Hutchings with immediate effect.
"Assistant Manager Frank Barlow will be taking charge of the first team on a caretaker basis, starting with next Sunday's trip to Tottenham Hotspur at White Hart Lane.
"The club wish to put on record their immense gratitude for Chris' efforts, in particular his contribution to the meteoric rise of Wigan Athletic over the past six and a half seasons."
Chairman Dave Whelan said: "Having been so close to the team and such a big part of the success, I felt Chris deserved to be given the chance to take over as No1 last summer and receive full backing from myself and the board to shape the team as he wished.
"However, it is crucial for the development of Wigan Athletic that we maintain our Premier League status this season and following results and performances in recent matches, we feel it is critical to act now."