League One Leyton Orient chairman Barry Hearn will mount a legal challenge over the future of the Olympic Stadium.
London Legacy Development Corporation (LLDC) has named West Ham United as the preferred bidder to move into the venue and said the process had been "robust, fair and transparent".
But Hearn told BBC London: "We believe the rules of the bidding process set out by the LLDC have not been followed.
"We are challenging them by asking the High Court to issue a judicial review."
In a statement LLDC said: "We have been notified that Leyton Orient have made the decision to issue proceedings for judicial review.
"Whilst this is disappointing, we believe that our processes have been robust, fair and transparent and that the challenge is misconceived."