The England hitman's partnership with Emmanuel Adebayor, who is set to receive a hostile reception from the Arsenal supporters, has been key to Tottenham's upturn in form, with the pair finding the net seven times since Adebayor signed on loan from Manchester City for the season.
"It's the big one, a massive game," Defoe said.
"Games like these are the ones you want to play in.
"I will definitely be ready for it. I'm sure the fans can't wait and we can't either.
"With the way that we are playing, we are confident. If we play like we did against Liverpool, with the same intensity, the same passing and the same movement that we did against them, then I can't see why we can't win the game."