Defoe had to be helped off after injuring ankle ligaments in yesterday’s Prem win at West Brom.
He will miss the clash with the Samba boys at Wembley on Wednesday.
And Spurs boss Andre Villas-Boas said: “I am happy apart from the setback to Jermain Defoe. He has rolled his ankle. We have to scan to make sure what the extent of the injury is.
“Hopefully there is not a lot of ligament damage. If there is, it could be up to two or three weeks to recover.”