Defoe had to be helped off after injuring ankle ligaments in yesterdayâ€™s Prem win at West Brom.
He will miss the clash with the Samba boys at Wembley on Wednesday.
And Spurs boss Andre Villas-Boas said: â€śI am happy apart from the setback to Jermain Defoe. He has rolled his ankle. We have to scan to make sure what the extent of the injury is.
â€śHopefully there is not a lot of ligament damage. If there is, it could be up to two or three weeks to recover.â€ť