Byrne believes his unfashionable Scunthorpe team are more than capable of pulling off a shock win over the holders.
"It will be a difficult night for us, come what may," said Byrne. "But if he sends down too many young lads, then who knows? This is a difficult place to play and once we are bang at it we will cause anyone all sorts of problems.
"We know that we are not a fashionable club but we also know we are a good team.
"We revel in being the underdogs but to us it does not matter because we know we are a good team and I cannot imagine there are many teams in the Championship that like coming here to play us.
"At the end of the day, everyone expects to turn us over and when they don't it is a bit of an eye-opener for them and we use that to our advantage.
"Although we would much rather be playing their first team, we know we will have a better opportunity to beat them if they rotate their squad."