"It will always be Juve-Roma, both for the players and the fans," the Giallorossi captain told Sky Italia. "It is a different game to all the others because, for some, Juve-Roma is more important than Roma-Lazio."
There is another rivalry on show in Turin this evening, as Totti is battling David Trezeguet to retain the Capocannoniere title.
"I have many more games at my disposal and hope I can get back to my best without all these niggling injuries that affect my form.
"If I return to the form of last term or the start of this season, I can challenge anyone."
Totti won the Golden Boot in 2007 as the top scorer in all European League competition, something Trezeguet has never managed.
"I don't think he's jealous! I know him as a person, he is very kind and we get on well.
"I am also friends with Gigi Buffon and sent him a text message of congratulations when his son was born. When we meet up I can give him some advice on dealing with babies, seeing as I have the clear advantage."