Di Maria has denied claims he sought to offend fans by grabbing his nether regions.
Ancelotti said: "We have to believe the player. He's given his explanation. If anyone thinks that it was a rude gesture, he's apologised. He's focused and I don't think he'll have any problems at the Bernabéu. That's my hope. These fans are always demanding but they're very fond of their players and I don't think he'll have any problems tomorrow.
"The fans understand him. It's possible that he made a mistake but the issue has to be finished with quickly because we have a lot of matches and we need him."
For breaking stories and all the great banter like us on Facebook: facebook.com/tribalfootball