During the 1-0 loss, Real coach Zinedine Zidane chose to change Isco for Karim Benzema as they went chasing a goal.
Isco angered teammates with his attitude upon the substitution, taking 45 seconds to leave the field.
At the final whistle, Ramos sought out Isco and Sport reports according to journalist José Luis Sánchez, rapped: "At Madrid, you have to run off the field.
"You have to show a better attitude."
Ramos was also angered by the approach of several teammates to the game, believing many failed to live up to expected standards.