Wolves boss Mick McCarthy has been asked to explain the ten changes to his team which faced Manchester United on Tuesday night. Wolves fans vented their frustration during the game, which was lost 3-0, although McCarthy defended his stance.
However, the Premier League have now got involved and want to know McCarthy's version of events.
A statement issued by the Premier League on Wednesday afternoon read: "The Premier League Board have written to Wolverhampton Wanderers to request their observations in relation to the team fielded in their League fixture against Manchester United.
"Once the observations are received the Board will decide whether any further action is warranted."
Although the Premier League's powers are wide-ranging, and include a points deduction in the worst-case scenario, it seems highly unlikely Wolves will receive anything worse than a rap over the knuckles.
McCarthy would also presumably point out he was balancing the demands of a congested fixture programme with a number of niggling injuries, which left him to make what he admitted was likely to prove a noteworthy decision.
"The injuries tipped the balance," said the former Republic of Ireland boss.
"I don't have a policy and I am not worried about repercussions from the Premier League.
"I said I would play my best team and that was the fittest, strongest team I could pick."
Disgruntled Wolves fans chanted "We want our money back", "Where is our first team?" and "£42 to watch the reserves" as they expressed their frustration.
"I understand the reaction of the fans and I knew these questions would be asked," McCarthy added.
"But my decisions will be judged on whether we are still in the Premier League."