The Premier League will regulate football's relationship with betting after it emerged junior matches can now be gambled on.
SBObet and 188bet, who are both licensed on the Isle of Man, offer live betting on matches at Premier League under-18 level and similar matches across Europe including the German Bundesliga under-17 league.
Both betting firms have a presence in the Premier League through tie-ups with several leading clubs.
Betting on junior matches does not contravene any rules, however the Premier League will examine the issue.
A Premier League spokesman told the Sunday Telegraph: "We are alarmed at these developments and are currently discussing this matter with our clubs and the government.
"What is clear is that this further highlights the need for the relationship between sport and the gambling industry to be properly regulated in order to protect the integrity of sport."
Professional Footballers Association chief executive Gordon Taylor said: "It is making our youngsters vulnerable, without a shadow of a doubt. Youngsters and gambling is a bad mix, and an unhealthy one."
A spokesman for 188bet told the newspaper that the company is committed to protecting "betting integrity" and stressed they were unaware of any concerns from the Premier League or PFA.
The spokesman said: "A key element of our betting governance includes setting lower bet limits on smaller markets, such as Premier League Academy games, to ensure that maximum individual bets and total bet volume is not of a size that would influence any sporting outcome."