Walker, who is said to be keen on a move to Manchester City, is in the squad to face West Ham on Friday.
Pochettino said: "All 21 players are focused to help achieve the things we want to achieve," "I am so calm. The players are calm. They know what happens inside.
"It's important that the fans know that the players are calm and we will see at the end of the season whether the rumours are true or not."
"The rotation between Danny Rose and Ben Davies, or Kyle and Trippier, you can see their performances."