The USL Pro Division club will host the Giallorossi on Wednesday, January 2 at Barker Family Stadium in Winter Park, Florida.
"It's obviously a fixture we're incredibly looking forward to," said Lions head coach Adrian Heath.
"We realize we're playing one of the biggest clubs in the world. We know it's going to be extremely difficult, but we just want to give a good account of ourselves."
Roma currently sits sixth on Italy's top flight table and boasts such names as Daniele De Rossi, Francesco Totti, Miralem Pjanic, Erik Lamela, Maarten Stekelenburg, Simone Perrotta, Pablo Osvaldo and USA international Michael Bradley.