Noble, who regularly takes penalties, strode up to take the first spot kick in the stadium.
He said: "It's a privilege to be the first West Ham player to kick a football inside the Olympic Stadium and as a lifelong West Ham fan it was great fun to film the video.
"Hopefully I will be one of those players walking out of the tunnel soaking in the atmosphere, with 54,000 West Ham flags and banners waving.
"The noise is going to be amazing and it's going to be a fantastic stadium to play in. 2016 just can't come quick enough."