Charlie Brooks, the company's UK spokesman, said: "It was an impact injury sustained when being challenged by the defender and Wayne has simply been unlucky. Nike and Wayne himself are positive there is no link between Wayne's injury and his boots."
Gerard Hartmann, an elite athletes' physiotherapist who has worked for Nike as a consultant, insisted the type of injury sustained by Rooney is nothing new.
"It's not the footwear but the demands placed on athletes, be they footballers or whoever," he told the Daily Mail.
"The twisting, turning and pivoting movements make football a very intense game and it's like anything - if you keep bending a wire hanger then it will be OK 20 times and then snap the 21st time.
"Suddenly we're aware of the metatarsal, but it's nothing new. Footballers have always had foot injuries but they are described as metatarsal injuries in a way that has made people believe it's a new phenomenon."