The Ecuadorian wing-back has missed United's last two games with a muscular injury.
"We played with the players available [against Sunderland]," Mourinho said.
"Not one single player stayed in Manchester because we were thinking about resting them, or because we were thinking about rotation or about Anderlecht. Not at all.
"These are the players available at the moment and, when I look to the others, I just expect Valencia to be ready to play and no more, I don't think. I don't think any one of the other boys will be ready to play."