Portugal suffered a major injury blow today after it was announced winger Nani would play no part in the World Cup after damaging his collarbone.
Nani's absence will disrupt the plans of Portugal boss Carlos Queiroz, who was counting on the 23-year-old following an impressive season at Old Trafford.
A statement on the Portuguese Football Federation's official website read: "Following the injury to the left clavicle suffered by Nani, a daily assessment of the damage and the completion of medical tests, we have concluded he is unable to be competitive.
"The medical file will now be presented to the medical department of Manchester United.
"In light of the report, the Portuguese Football Federation, taking into account the FIFA regulations for the 2010 World Cup, will call-up Ruben Amorim as a replacement."