Balotelli, who hopes to start the Euro quarter-final clash for Italy in Kiev, suffered pain in his back as he left the pitch.
Italian doctor Enrico Castellacci said: "Balotelli got some small pain in his back but will be fit.
"Chiellini is improving a lot but he will not be ready for England. Motta is training and we will assess him. We do not know if he can face England."