The Magpies have now extended their winless run to four matches after Martyn Woolford netted a double to down them at Glanford Park but Hughton is remaining calm in regards to being offered the job permanently.
"It will have no effect at all," he said of the defeat.
"As far as I know they (the talks) are still going ahead. I'm still very confident the situation hasn't changed."
Hughton did, however, concede that Newcastle were simply not good enough and let Scunthorpe dictate terms too much.
"The game plan was to come here and do well," he added.
"On the balance of play and chances it's a game we should have won, but we have to do better.
"They were able to turn us at times, they were always a threat and had a lot of energy in the side and at the clinical moments they got the goals.
"We didn't defend moments well enough and that enabled them to get the goals that they did. On the balance of play it's a game that we should have won.
"I think this is a pressure on us. We are a side in this division that everybody expects to do well and certainly our own supporters expect us to do well and that's the pressure that we carry with us in every game. That's a pressure that we've got to live with."