Agger was withdrawn shortly after the hour mark during a dramatic 4-3 victory over Swansea last Sunday - and Brendan Rodgers confirmed afterwards that injury had not forced the substitution.
Ahead of the trip to Southampton, Rodgers said: "There has been a lot of spotlight, unfairly, on Daniel this week. What happened to Daniel was no different to what happened to any player.
"Just because it's a defender, it maybe gets highlighted a bit more. But we made the change and we won the game. It's no different to a winger coming off, or a striker or a midfield player.
"I don't judge players on the status of where they are in the group - it's what I think the game needs at a certain time. We made the change and Dan has taken it like the man he is.
"He has been outstanding in training this week and he will play. He will play on Saturday, no question about that."
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