Juve need four points from their next two games to qualify for the last 16 of the Champions League.
Referring to Chelsea's 3-2 win over Shakhtar Donetsk, Marchisio said: “A 2-2 draw would have been a better result for us, but we required a victory and that’s exactly what we got.
“Our ambitions haven’t changed. With desire and concentration, we’ll give it a good go against Chelsea – knowing that we’ve also got the Shakhtar game remaining.
“We want to go through.”