Holland coach Guus Hiddink says Aston Villa captain Ron Vlaar's latest injury is a fresh one for the defender.
Vlaar pulled up in the first half and was replaced by Lazio's Stefan de Vrij in the defeat to Mexico.
Karim Rekik has been called up to the squad to replace Vlaar, but the defender, on loan at PSV from Manchester City, only has one previous cap as a substitute in a 2-0 defeat by France.
Hiddink confirmed on Wednesday night to Voetbal Primeur, "It does not look good, Ron is having trouble with his calf. It's not the same place as last time that was totally different."
