Guardiola plans to have Carlos Puyol and Eric Abidal at peak condition for the Wembley date.
Puyol was deemed unfit to start against Espanyol, but Guardiola says he will be ready to play at Wembley.
"He was out for three months (recently) and the doctors decided he wasn't in shape to play today, but he is building up his fitness and will feature more in the next few games," he said.
Left-back Abidal came on to play the last 20 minutes and Guardiola revealed the Frenchman, who has recently returned from an operation to remove a tumour from his liver in March, could also be ready to play in the Champions League final.
"Abi had a few minutes the other day (in the Champions League semi-final second leg against Madrid) and played for longer today," he said.
"We hope he can gradually play more and more and build up his fitness for the final."